Comparison of induced corpora lutea from prepuberal gilts and spontaneous corpora lutea from mature gilts: in vitro progesterone production.

@article{Kineman1987ComparisonOI,
  title={Comparison of induced corpora lutea from prepuberal gilts and spontaneous corpora lutea from mature gilts: in vitro progesterone production.},
  author={R D Kineman and George B. Rampacek and Robert R. Kraeling and N A Fiorello-Stocks and Robert L. Wilson},
  journal={Journal of animal science},
  year={1987},
  volume={64 2},
  pages={526-32}
}
Prepuberal (P) gilts were induced to ovulate with pregnant mare serum gonadotropin followed 72 h later by human chorionic gonadotropin (hCG). Three P gilts and three mature (M) gilts each were ovariectomized on d 10, 14, 18, 22 and 26 (d 0 = day of hCG for P gilts and onset of estrus for M gilts). Gilts ovariectomized on d 14, 18, 22 and 26 were hysterectomized on d 6 to ensure maintenance of the corpora lutea (CL). Two to five grams of minced luteal tissue were dispersed using collagenase and… CONTINUE READING

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Two to five grams of minced luteal tissue were dispersed using collagenase and hyaluronidase in HEPES buffered salt solution supplemented with glucose and bovine serum albumin .
Comparison of induced corpora lutea from prepuberal gilts and spontaneous corpora lutea from mature gilts : in vitro progesterone production .
Comparison of induced corpora lutea from prepuberal gilts and spontaneous corpora lutea from mature gilts : in vitro progesterone production .
Comparison of induced corpora lutea from prepuberal gilts and spontaneous corpora lutea from mature gilts : in vitro progesterone production .
Comparison of induced corpora lutea from prepuberal gilts and spontaneous corpora lutea from mature gilts : in vitro progesterone production .
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