Comparison of in vitro cell cytotoxic assays for tumor necrosis factor.

  title={Comparison of in vitro cell cytotoxic assays for tumor necrosis factor.},
  author={David A. Flick and George E. Gifford},
  journal={Journal of immunological methods},
  volume={68 1-2},
Four published in vitro assays which measure cell cytotoxicity were compared utilizing murine tumor necrosis factor. These included determination of residual cell number by crystal violet staining in the presence and absence of actinomycin D, lack of viability as determined by neutral red uptake, and [3H]thymidine release in cytotoxin treated L929 cells. Treatment of cells with actinomycin D followed by crystal violet staining was the most sensitive method measured. However, addition of… CONTINUE READING

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