Comparison of in vitro and in vivo reference genes for internal standardization of real-time PCR data.

@article{Gilsbach2006ComparisonOI,
  title={Comparison of in vitro and in vivo reference genes for internal standardization of real-time PCR data.},
  author={Ralf Gilsbach and Mina Kouta and Heinz B{\"o}nisch and Michael Br{\"u}ss},
  journal={BioTechniques},
  year={2006},
  volume={40 2},
  pages={
          173-7
        }
}
Real-time PCR is a powerful technique for gene expression studies, which have become increasingly important in a large number of clinical and scientific fields. The significance of the obtained results strongly depends on the normalization of the data to compensate for differences between the samples. The most widely used approach is to use endogenous reference genes (housekeeping genes) as internal standards. This approach is controversially discussed in the literature because none of the… CONTINUE READING

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