Comparison of immune responses induced in mice by vaccination with DNA vaccine constructs expressing mycobacterial antigen 85A and interleukin-21 and Bacillus Galmette-Guérin.

@article{Dou2008ComparisonOI,
  title={Comparison of immune responses induced in mice by vaccination with DNA vaccine constructs expressing mycobacterial antigen 85A and interleukin-21 and Bacillus Galmette-Gu{\'e}rin.},
  author={Jun Dou and Quan Yong Tang and Fengshu Zhao and Lili Chu and Junsong Chen and Minggang Cao and Chunsheng Liu and Yongfang Wang and Yating Li and Jin Long Li},
  journal={Immunological investigations},
  year={2008},
  volume={37 2},
  pages={113-27}
}
In this paper, we addressed the immune adjuvant effects of interleukin(IL)-21 on DNA vaccine constructs expressing mycobacterium tuberculosis (TB) Ag85A and compared immune responses induced in mice inoculated DNA vaccine constructs expressing Ag85A and IL-21 with mice inoculated DNA vaccine constructs expressing Ag85A alone or Bacillus Galmette-Guérin(BCG.). In this experiment, the gene of IL-21 was firstly amplified from plasmid pcDNA3.1-mIL21 by PCR and cloned into the plasmid pRSC, forming… CONTINUE READING