Comparison of high performance liquid chromatography, radio immunoassay and electrochemiluminescence immunoassay for quantification of serum 25 hydroxy vitamin D.

@article{Jafri2011ComparisonOH,
  title={Comparison of high performance liquid chromatography, radio immunoassay and electrochemiluminescence immunoassay for quantification of serum 25 hydroxy vitamin D.},
  author={Lena Jafri and Aysha Habib Khan and Anwar Ali Siddiqui and Shamim Mushtaq and Romania Iqbal and Farooq Ghani and Imran Siddiqui},
  journal={Clinical biochemistry},
  year={2011},
  volume={44 10-11},
  pages={864-8}
}
OBJECTIVE To compare the performance of radioimmunoassay (RIA) with high-performance liquid chromatography (HPLC) and electrochemiluminescence (ECLIA) for the quantification of vitamin D (25OHD). METHODS HPLC method for the determination of 25OHD in human biological samples was developed and compared in terms of accuracy and precision with a commercially… CONTINUE READING