Comparison of high-performance liquid chromatography and bioassay for the determination of 5-fluorocytosine in serum.

  • Joerg Huelsewede
  • Published 1994 in
    Zentralblatt für Bakteriologie : international…

Abstract

An HPLC method using a reverse phase system, an isocratic mobile phase and simple protein precipitation and a plate diffusion bioassay using an amphotericin B resistant strain of Candida pseudotropicalis for the measurement of 5-fluorocytosine in serum were compared. Both methods permit a determination of 5-fluorocytosine in sera also in the presence of amphothericin B. The correlation between bioassay and HPLC runs was found to be r = 0.96. Both methods are useful for monitoring the serum level of 5-fluorocytosine in clinical routine.

Cite this paper

@article{Huelsewede1994ComparisonOH, title={Comparison of high-performance liquid chromatography and bioassay for the determination of 5-fluorocytosine in serum.}, author={Joerg Huelsewede}, journal={Zentralblatt für Bakteriologie : international journal of medical microbiology}, year={1994}, volume={281 4}, pages={513-8} }