Comparison of high performance liquid chromatography and stereological image analysis for the quantitation of eumelanins and pheomelanins in melanoma cells.

@article{Donois1998ComparisonOH,
  title={Comparison of high performance liquid chromatography and stereological image analysis for the quantitation of eumelanins and pheomelanins in melanoma cells.},
  author={E. Donois and V{\'e}ronique Del Marmol and Kazumasa Wakamatsu and Susumu Ito and G Ghanem and Jean Etienne Surl{\`e}ve-Bazeille},
  journal={Pigment cell research},
  year={1998},
  volume={11 2},
  pages={
          86-93
        }
}
The aim of the study was to compare two methods quantifying eumelanins and pheomelanins, pigments synthesized by melanocytes. One is based on the high performance liquid chromatography (HPLC) quantitation of specific degradation products of each melanin type. The other requires image analysis, transmission electron microscopy (TEM), and stereology. This study was carried out in cultured human melanoma cells and for each line, melanins were measured by HPLC and cells were fixed and embedded as… CONTINUE READING
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