Comparison of gene transcription in cloned bovine embryos produced by different nuclear transfer techniques.

@article{Daniels2001ComparisonOG,
  title={Comparison of gene transcription in cloned bovine embryos produced by different nuclear transfer techniques.},
  author={Rob Daniels and Vanessa J Hall and Andrew J. French and Natasha A Korfiatis and Alan O. Trounson},
  journal={Molecular reproduction and development},
  year={2001},
  volume={60 3},
  pages={281-8}
}
The efficiency of animal production using cloning technology is still relatively low and research to determine a more efficient nuclear transfer procedure is ongoing. One approach which may be informative in assessing the viability of nuclear transfer embryos is the analysis of embryonic gene expression. Using RT-PCR techniques we have previously detected the aberrant expression of FGF4, FGFr2 and IL6 in a significant proportion of bovine granulosa cell-derived nuclear transfer embryos, which… CONTINUE READING