Comparison of freeze-blowing and funnel-freezing of rat brain for the measurement of cerebral glucose concentration in vivo.

@article{Mies1991ComparisonOF,
  title={Comparison of freeze-blowing and funnel-freezing of rat brain for the measurement of cerebral glucose concentration in vivo.},
  author={Guenter Mies and Nildris Cruz and Louis Sokoloff},
  journal={Journal of neurochemistry},
  year={1991},
  volume={56 5},
  pages={1673-6}
}
The efficacy of funnel-freezing of rat brain to inactivate metabolic processes and preserve in vivo tissue glucose concentration was validated by comparing the results obtained by funnel-freezing with those obtained with freeze-blowing of brain. The arterial plasma glucose level was clamped at 9 mM in halothane-anesthetized rats to produce identical glucose… CONTINUE READING