Comparison of four serum-free, cytokine-free media for analysis of endogenous erythroid colony growth in polycythemia vera and essential thrombocythemia.

@article{Dobo2001ComparisonOF,
  title={Comparison of four serum-free, cytokine-free media for analysis of endogenous erythroid colony growth in polycythemia vera and essential thrombocythemia.},
  author={Ir{\`e}ne Dobo and Pascal Mossuz and Lydia Campos and François Girodon and Annie All{\'e}graud and V{\'e}ronique Latger-Cannard and Nathalie Boiret and Danielle Pineau and Ellen Wunder and Marc Zandecki and Vincent Praloran and Sylvie Hermouet},
  journal={The hematology journal : the official journal of the European Haematology Association},
  year={2001},
  volume={2 6},
  pages={
          396-403
        }
}
INTRODUCTION The assay of endogenous erythroid colony formation (EEC), a characteristic of polycythemia vera and essential thrombocythemia, is not standardized. In this multicentric study, we tested four semisolid, serum-free, cytokine-free media based on either methylcellulose (M1, M2) or collagen (C1, C2) commercialized for the EEC assay. MATERIALS AND METHODS Bone marrow mononuclear cells (BMMC) from 73 individuals (62 patients with either polycythemia vera (26), essential thrombocythemia… CONTINUE READING
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