Comparison of four DNA extraction methods for comprehensive assessment of 16S rRNA bacterial diversity in marine biofilms using high-throughput sequencing.

@article{Corcoll2017ComparisonOF,
  title={Comparison of four DNA extraction methods for comprehensive assessment of 16S rRNA bacterial diversity in marine biofilms using high-throughput sequencing.},
  author={Nat{\`a}lia Corcoll and Tobias {\"O}sterlund and Lucas Sinclair and Alexander Eiler and Erik Kristiansson and Thomas Backhaus and Karl Martin Eriksson},
  journal={FEMS microbiology letters},
  year={2017},
  volume={364 14}
}
High-throughput DNA sequencing technologies are increasingly used for the metagenomic characterisation of microbial biodiversity. However, basic issues, such as the choice of an appropriate DNA extraction method, are still not resolved for non-model microbial communities. This study evaluates four commonly used DNA extraction methods for marine periphyton biofilms in terms of DNA yield, efficiency, purity, integrity and resulting 16S rRNA bacterial diversity. Among the tested methods, the Plant… CONTINUE READING