Comparison of fluorescent stains: relative photostability and differential staining of proteins in two-dimensional gels.

@article{Smejkal2004ComparisonOF,
  title={Comparison of fluorescent stains: relative photostability and differential staining of proteins in two-dimensional gels.},
  author={Gary B. Smejkal and Myra H Robinson and Alexander Lazarev},
  journal={Electrophoresis},
  year={2004},
  volume={25 15},
  pages={2511-9}
}
The fluorescence of proteins stained with Deep Purple and SYPRO Ruby was measured over a time course of UV transillumination to determine the relative photostability of each stain. Mean spot fluorescence (n = 200 matched spots) in gels stained with Deep Purple decreased 27% following 2 min of UV transillumination, compared to SYPRO Ruby, which decreased 17%. After 19 min, an 83% decrease in Deep Purple fluorescence was observed, compared to 44% for SYPRO Ruby. By interpolation, the half-life of… CONTINUE READING

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