Comparison of fluorescence-based semi-automated genotyping of multiple microsatellite loci with autoradiographic techniques.

@article{Schwengel1994ComparisonOF,
  title={Comparison of fluorescence-based semi-automated genotyping of multiple microsatellite loci with autoradiographic techniques.},
  author={Deborah A Schwengel and Anne E. Jedlicka and Elizabeth Nanthakumar and J. L. Weber and Roy C Levitt},
  journal={Genomics},
  year={1994},
  volume={22 1},
  pages={46-54}
}
The practical application of highly efficient fluorescence-based methods for the semi-automated genotyping of polymerase chain reaction-based microsatellite markers will depend on the development of robust protocols that provide accurate and reproducible data. In the present report we compare the accuracy of a fluorescence-based protocol with a benchmark radiolabeling method that depends on a known sequence ladder or amplified DNA from reference individuals for sizing by autoradiography. Three… CONTINUE READING

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