Comparison of fathead minnow ovary explant and H295R cell-based steroidogenesis assays for identifying endocrine-active chemicals.

@article{Villeneuve2007ComparisonOF,
  title={Comparison of fathead minnow ovary explant and H295R cell-based steroidogenesis assays for identifying endocrine-active chemicals.},
  author={Daniel L. Villeneuve and Gerald T. Ankley and Elizabeth A. Makynen and Lindsey S Blake and Katie J Greene and Eric B Higley and John L. Newsted and John Paul Giesy and Markus Hecker},
  journal={Ecotoxicology and environmental safety},
  year={2007},
  volume={68 1},
  pages={20-32}
}
An in vitro steroidogenesis assay using H295R human adenocarcinoma cells has been suggested as a possible alternative to gonad explant assays for use as a Tier I screening assay to detect endocrine active chemicals capable of modulating steroid hormone synthesis. This study is one of the first to investigate the utility of the H295R assay for predicting effects and/or understanding mechanisms of action across species and tissues. Six chemicals, including one selective aromatase inhibitor… CONTINUE READING

Citations

Publications citing this paper.
Showing 1-10 of 27 extracted citations

References

Publications referenced by this paper.
Showing 1-10 of 58 references

Evaluation of the the antiandrogenic properties of vinclozolin in the fathead minnow

D. Martinovic, Blake. L.S, +6 authors G. T. Ankley
Society of Environmental Toxicology and Chemistry North America, • 2006
View 4 Excerpts
Highly Influenced

Effects of three fungicides on fathead minnow ovarian steroid synthesis in vitro

D. L. Villeneuve, E. A. Makynen, G. T. Ankley
Society of Environmental Toxicology and Chemistry, North America, 26th Annual Meeting, November • 2005
View 11 Excerpts
Highly Influenced

Human adrenocarcinoma (H295R) cell system for rapid testing of chemical effects on steroidogenesis

M. Hecker, J. L. Newsted, +4 authors J. P. Giesy
Toxicol. Appl. Pharmacol • 2006
View 19 Excerpts
Highly Influenced

Vertebrate Endocrinology, third ed

D. O. Norris
1997
View 5 Excerpts
Highly Influenced

Protocol for measuring in vitro steroid production by fish gonadal tissue

M. E. McMaster, K. R. Munkittrick, J. J. Jardine, R. D. Robinson, G. J. Van Der Kraak
Canadian Technical Report of Fisheries and Aquatic Sciences • 1995
View 4 Excerpts
Highly Influenced

Comparison of pesticides in eight US urban streams

R. S. Hoffman, P. D. Capel, S. J. Larson
Environ. Toxicol. Chem • 2000
View 3 Excerpts
Highly Influenced

Pesticides in streams of the United States — Initial results from the National Water - Quality assessment program

S. J. Larson, R. J. Gilliom, P. D. Capel
USGS Water - Resources Investigations Report 98 - 4222 • 1999
View 2 Excerpts
Highly Influenced

Similar Papers

Loading similar papers…