Comparison of extraction methods for insulin-like growth factor-I in rat serum.

@article{Crawford1992ComparisonOE,
  title={Comparison of extraction methods for insulin-like growth factor-I in rat serum.},
  author={Bronwyn A. L. Crawford and Janet L. Martin and Christopher J. Howe and David J Handelsman and Robert Charles Baxter},
  journal={The Journal of endocrinology},
  year={1992},
  volume={134 2},
  pages={
          169-76
        }
}
This study was undertaken to compare various extraction methods for insulin-like growth factor-binding proteins (IGFBPs) from insulin-like growth factor-I (IGF-I) in rat serum systematically, before measurement of IGF-I by radioimmunoassay (RIA). The values obtained in the IGF-I RIA following acid-ethanol (AE), acid-ethanol cryoprecipitation (AEC) and formic acid-acetone (FA) extraction methods were compared with the IGF-I values obtained following high-performance liquid chromatography (HPLC… CONTINUE READING

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