Comparison of extraction methods and assay validation for salmon insulin-like growth factor-I using commercially available components.

@article{Shimizu2000ComparisonOE,
  title={Comparison of extraction methods and assay validation for salmon insulin-like growth factor-I using commercially available components.},
  author={Munetaka Shimizu and Penny Swanson and Haruhisa Fukada and Akihiko Hara and Walton W. Dickhoff},
  journal={General and comparative endocrinology},
  year={2000},
  volume={119 1},
  pages={26-36}
}
Insulin-like growth factor-binding proteins (IGFBPs) may interfere with accurate measurement of plasma IGFs in radioimmunoassay (RIA). Although several simplified extraction methods for IGFs have been developed, these methods are not always validated for differing physiological states, developmental stages, and animal species. For teleost fish, neither the necessity of plasma extraction nor the validity of extraction methods for IGF RIA is widely established. We systematically examined the… CONTINUE READING