Comparison of ethanol versus formalin fixation on preservation of histology and RNA in laser capture microdissected brain tissues.

@article{Su2004ComparisonOE,
  title={Comparison of ethanol versus formalin fixation on preservation of histology and RNA in laser capture microdissected brain tissues.},
  author={Jack Meng Fen Su and Laszlo Perlaky and Xiao-Nan Li and Hon-chiu E. Leung and Barbara C Antalffy and Dawna Armstrong and Ching C. Lau},
  journal={Brain pathology},
  year={2004},
  volume={14 2},
  pages={175-82}
}
Although RNA can be retrieved from formalin-fixed, paraffin-embedded (FFPE) tissues, the yield is low, and the RNA is fragmented. Recent advances in gene expression profiling underscore the importance of identifying a fixative that preserves histology and mRNA. We demonstrated that, for immersion fixation of brains, 70% ethanol is superior to formalin for mRNA preservation. RNA yield from ethanol-fixed tissues was 70% of the yield from fresh frozen specimens, but only a negligible quantity was… CONTINUE READING