Comparison of estimates of ruminal protein degradation by in vitro and in situ methods.

@article{Broderick1988ComparisonOE,
  title={Comparison of estimates of ruminal protein degradation by in vitro and in situ methods.},
  author={Glen A. Broderick and Robert J. Wallace and E R Orskov and Lory Hansen},
  journal={Journal of animal science},
  year={1988},
  volume={66 7},
  pages={1739-45}
}
Ruminal degradation of eight soluble proteins and 10 protein meals was determined using three methods: 1) an inhibitor in vitro system (IIV), to which inhibitors are added to prevent metabolism of protein degradation products, 2) in situ incubations in nylon bags and 3) in vitro NH3 production in typical ruminal inoculum. In vitro NH3 production rate from different proteins was not related to in situ or IIV degradation rate. Degradation rates for soluble proteins by IIV ranged from .103 to .813… CONTINUE READING