Comparison of enzyme immunoassay and reverse transcriptase PCR for identification of serotype G9 rotaviruses.

@article{Coulson1999ComparisonOE,
  title={Comparison of enzyme immunoassay and reverse transcriptase PCR for identification of serotype G9 rotaviruses.},
  author={Barbara S Coulson and Jon R. Gentsch and Bimal Kumar Das and M K Bhan and Roger I. Glass},
  journal={Journal of clinical microbiology},
  year={1999},
  volume={37 10},
  pages={3187-93}
}
While only four globally important rotavirus G serotypes (1 to 4) have been documented, many studies suggest that serotype G9 viruses may be widely distributed and more important than previously recognized. We have evaluated 10 serotype G9 rotavirus-neutralizing monoclonal antibodies (MAbs) directed to VP7, which bound by direct enzyme immunoassay (EIA) to P1A[8], G9 rotaviruses F45, WI61, and AU32, for their ability to recognize the New Delhi G9 rotavirus 116E. Only one MAb (MAb F45:1) bound… CONTINUE READING

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