Comparison of electro-fusion and intracytoplasmic nuclear injection methods in pig cloning.

@article{Kurome2003ComparisonOE,
  title={Comparison of electro-fusion and intracytoplasmic nuclear injection methods in pig cloning.},
  author={Mayuko Kurome and Tatsuya Fujimura and Hiroshi Murakami and Youichi Takahagi and Naohiro Wako and Takashi Ochiai and K{\^o}ji Miyazaki and Hiroshi Nagashima},
  journal={Cloning and stem cells},
  year={2003},
  volume={5 4},
  pages={367-78}
}
This paper methodologically compares the electro-fusion (EF) and intracytoplasmic injection (ICI) methods, as well as simultaneous fusion/activation (SA) and delayed activation (DA), in somatic nuclear transfer in pigs using fetal fibroblast cells. Comparison of the remodeling pattern of donor nuclei after nuclear transfer by ICI or EF showed that a high rate (80-100%) of premature chromosome condensation occurred in both cases whether or not Ca2+ was present in the fusion medium. Formation of… CONTINUE READING

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