Comparison of effects of zona drilling by non-contact infrared laser or acid Tyrode's on the development of human biopsied embryos as revealed by blastomere viability, cytoskeletal analysis and molecular cytogenetics.

@article{Chatzimeletiou2005ComparisonOE,
  title={Comparison of effects of zona drilling by non-contact infrared laser or acid Tyrode's on the development of human biopsied embryos as revealed by blastomere viability, cytoskeletal analysis and molecular cytogenetics.},
  author={Katerina Chatzimeletiou and Ewan E. Morrison and Yannis Panagiotidis and Nikos M. Prapas and Yannis M. Prapas and Anthony J. Rutherford and Gedis J. Grudzinskas and Alan H. Handyside},
  journal={Reproductive biomedicine online},
  year={2005},
  volume={11 6},
  pages={
          697-710
        }
}
Use of a non-contact infrared laser (IRL) or acid Tyrode's for zona drilling before embryo biopsy was compared by assessing blastomere viability using various fluorescent markers or culture of the single biopsied blastomere, and, by cytoskeletal and molecular cytogenetic analysis of the biopsied embryos following culture to the blastocyst stage. There was no significant difference in the proportion of biopsied embryos that showed no damage in both the biopsied blastomere and in the remaining… CONTINUE READING
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