Comparison of direct electron microscopy, immune electron microscopy, and rotavirus enzyme-linked immunosorbent assay for detection of gastroenteritis viruses in children.

@article{Brandt1981ComparisonOD,
  title={Comparison of direct electron microscopy, immune electron microscopy, and rotavirus enzyme-linked immunosorbent assay for detection of gastroenteritis viruses in children.},
  author={Carl D Brandt and Hyun Wha Kim and William J. Rodriguez and Lee J. Thomas and Robert Yolken and Julita O Arrobio and Albert Z. Kapikian and Robert H Parrott and Robert M. Chanock},
  journal={Journal of clinical microbiology},
  year={1981},
  volume={13 5},
  pages={976-81}
}
An approximate 10% suspension in water of the first available stool sample from 411 infants and young children with acute gastroenteritis was examined by electron microscopy (EM) after 2 min of negative staining. This procedure enabled the detection of 88% of the 199 rotavirus infections, all of the 22 adenovirus infections, and 47% of the 15 approximately 27-nm virus infections ultimately detected by a combination of techniques, including immune electron microscopy (IEM) and rotavirus enzyme… CONTINUE READING
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