Comparison of direct and membrane feeding methods to infect Anopheles arabiensis with Plasmodium falciparum.

@article{AwonoAmbn2001ComparisonOD,
  title={Comparison of direct and membrane feeding methods to infect Anopheles arabiensis with Plasmodium falciparum.},
  author={Herman-Parfait Awono-Amb{\'e}n{\'e} and Lamine Diawara and Vincent Robert},
  journal={The American journal of tropical medicine and hygiene},
  year={2001},
  volume={64 1-2},
  pages={32-4}
}
Two standard methods are available to infect mosquitoes with malaria parasites: direct feeding through the skin of the gametocyte carrier, and membrane feeding. Anopheles arabiensis collected at larval stages and reared in an insectary were fed in parallel by feeding on Plasmodium falciparum gametocyte carriers and by membrane feeding on venous blood of the same gametocyte carriers. Infection of mosquitoes was assessed at Day 7 post bloodmeal by oocyst count of the mosquito midguts. The… CONTINUE READING