Comparison of digestion protocols for microgram quantities of enriched protein samples.

@article{Hervey2007ComparisonOD,
  title={Comparison of digestion protocols for microgram quantities of enriched protein samples.},
  author={W Judson Hervey and Michael B. Strader and Gregory B. Hurst},
  journal={Journal of proteome research},
  year={2007},
  volume={6 8},
  pages={3054-61}
}
Standard biochemical techniques that are used for protein enrichments, such as affinity isolation and density gradient centrifugation, frequently yield high-nanogram to low-microgram quantities at a significant expenditure of resources and time. The characterization of selected protein enrichments by the "shotgun" mass spectrometry approach is often compromised by the lack of effective and efficient in-solution proteolysis protocols specifically tailored for these small quantities of proteins… CONTINUE READING
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