Comparison of different methods for DNA-free RNA isolation from SK-N-MC neuroblastoma

@inproceedings{Tavares2010ComparisonOD,
  title={Comparison of different methods for DNA-free RNA isolation from SK-N-MC neuroblastoma},
  author={Luc{\'e}lia Tavares and Paula M. Alves and Ricardo Boavida Ferreira and Cl{\'a}udia N Santos},
  booktitle={BMC Research Notes},
  year={2010}
}
RNA quality and quantity are important factors for ensuring the accuracy of gene expression analysis and other RNA-based downstream applications. Extraction of high quality nucleic acids is difficult from neuronal cells and brain tissues as they are particularly rich in lipids. In addition, most common RNA extraction methods are phenol-based, resulting in RNA that may be incompatible with downstream applications such as gene expression. In this work, a comparative analysis of the RNA quality… CONTINUE READING

From This Paper

Figures, tables, and topics from this paper.

Citations

Publications citing this paper.
Showing 1-8 of 8 extracted citations

DNase concentration assay to obtain DNA-free RNA from sugarcane leaves.

Genetics and molecular research : GMR • 2016
View 3 Excerpts

References

Publications referenced by this paper.
Showing 1-10 of 15 references

DNA, RNA, and Protein Extraction: The Past and The Present

Journal of biomedicine & biotechnology • 2009
View 1 Excerpt

harbors an asymmetric bidirectional promoter but not internal ribosome entry site in vitro

J Sambrook, EF Fritsch, T Maniatis
Gene • 2009

Epigenetic regulation of human alpha1d-adrenergic receptor gene expression: a role for DNA methylation in Sp1-dependent regulation.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology • 2007
View 1 Excerpt

Identification and characterization of human NR4A2 polymorphisms in attention deficit hyperactivity disorder.

American journal of medical genetics. Part B, Neuropsychiatric genetics : the official publication of the International Society of Psychiatric Genetics • 2005
View 1 Excerpt

Nucleic acid-free matrix: Regeneration of DNA binding columns

KH Esser, WH Marx, T Lisowsky
Biotechniques • 2005
View 1 Excerpt