Comparison of different methods for DNA-free RNA isolation from SK-N-MC neuroblastoma

  title={Comparison of different methods for DNA-free RNA isolation from SK-N-MC neuroblastoma},
  author={Luc{\'e}lia Tavares and Paula M. Alves and Ricardo Boavida Ferreira and Cl{\'a}udia N Santos},
  booktitle={BMC Research Notes},
RNA quality and quantity are important factors for ensuring the accuracy of gene expression analysis and other RNA-based downstream applications. Extraction of high quality nucleic acids is difficult from neuronal cells and brain tissues as they are particularly rich in lipids. In addition, most common RNA extraction methods are phenol-based, resulting in RNA that may be incompatible with downstream applications such as gene expression. In this work, a comparative analysis of the RNA quality… CONTINUE READING

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