Comparison of detection of antibody to the acquired immune deficiency syndrome virus by enzyme immunoassay, immunofluorescence, and Western blot methods.

@article{Gallo1986ComparisonOD,
  title={Comparison of detection of antibody to the acquired immune deficiency syndrome virus by enzyme immunoassay, immunofluorescence, and Western blot methods.},
  author={Dana Gallo and J L Diggs and G R Shell and Peter J. Dailey and Malka Hoffman and John L. Riggs},
  journal={Journal of clinical microbiology},
  year={1986},
  volume={23 6},
  pages={1049-51}
}
There was 100% agreement between enzyme immunoassay (EIA) (Abbott Laboratories), Western blot, and indirect immunofluorescence (IF) when these three methods were used to measure antibody to the acquired immune deficiency syndrome (AIDS) virus in sera from 142 high-risk individuals, indicating that IF was a sensitive alternative method for detecting antibody to this agent. Thirty-two (64%) of 50 EIA-positive plasma specimens from a blood bank and 6 (21%) of 28 EIA-positive sera from alternative… CONTINUE READING

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