Comparison of cytochrome P450 concentrations and metabolic activities in porcine hepatic microsomes prepared with two different methods.

@article{Rasmussen2011ComparisonOC,
  title={Comparison of cytochrome P450 concentrations and metabolic activities in porcine hepatic microsomes prepared with two different methods.},
  author={Martin Kr{\o}yer Rasmussen and Britten Ekstrand and Galia Zamaratskaia},
  journal={Toxicology in vitro : an international journal published in association with BIBRA},
  year={2011},
  volume={25 1},
  pages={
          343-6
        }
}
In the present study, porcine liver microsomes prepared by a conventional ultracentrifugation method were compared with microsomes prepared by a calcium aggregation method. Protein concentrations and activities of several cytochrome P450 enzymes were measured. It was concluded that using a calcium aggregation method for microsome preparation resulted in lower activities of porcine 7-ethoxyresorufin O-deethylase (EROD), 7-methoxyresorufin O-demethylase (MROD), 7-pentoxyresorufin O-depentylase… CONTINUE READING
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