Comparison of conventional RT-PCR, reverse-transcription loop-mediated isothermal amplification, and SYBR green I-based real-time RT-PCR in the rapid detection of bovine viral diarrhea virus nucleotide in contaminated commercial bovine sera batches.

@article{Zhang2014ComparisonOC,
  title={Comparison of conventional RT-PCR, reverse-transcription loop-mediated isothermal amplification, and SYBR green I-based real-time RT-PCR in the rapid detection of bovine viral diarrhea virus nucleotide in contaminated commercial bovine sera batches.},
  author={Shu-qin Zhang and Bin Tan and Peng Li and Feng-Xue Wang and Li Guo and Yong Yang and Nan Sun and Hongwei Zhu and Yongjun Wen and Shipeng Cheng},
  journal={Journal of virological methods},
  year={2014},
  volume={207},
  pages={
          204-9
        }
}
Bovine viral diarrhea virus (BVDV) can contaminate biological products produced in bovine or porcine cells or manufactured using bovine sera. A rapid, specific, sensitive, and practical method of detecting BVDV in bio-products is needed. The purpose of this study was to compare three assays with respect to their ability to accurately detect BVDV in biological samples, namely reverse-transcription loop-mediated isothermal amplification (RT-LAMP), SYBR green I-based real-time RT-PCR, and… CONTINUE READING
BETA

References

Publications referenced by this paper.
SHOWING 1-10 OF 29 REFERENCES

in nonpregnant female alpacas ( Vicugna pacos )

  • H. S. Cho, N. Y. Park
  • 2014

Development of a SYBR green I based real - time RT - PCR assay for detection and quantification of bovine coronavirus

  • F. N. Almajhdi
  • Mol . Cell . Probes
  • 2011
2 Excerpts

Similar Papers

Loading similar papers…