Comparison of commercial DNA preparation kits for the detection of Brucellae in tissue using quantitative real-time PCR

@inproceedings{Tomaso2010ComparisonOC,
  title={Comparison of commercial DNA preparation kits for the detection of Brucellae in tissue using quantitative real-time PCR},
  author={Herbert Tomaso and Mireille M. Kattar and Meike Eickhoff and Ulrich Wernery and Sascha Al Dahouk and Eberhard Straube and Heinrich Neubauer and Holger Christian Scholz},
  booktitle={BMC infectious diseases},
  year={2010}
}
BACKGROUND The detection of Brucellae in tissue specimens using PCR assays is difficult because the amount of bacteria is usually low. Therefore, optimised DNA extraction methods are critical. The aim of this study was to assess the performance of commercial kits for the extraction of Brucella DNA. METHODS Five kits were evaluated using clinical specimens: QIAamp DNA Mini Kit (QIAGEN), peqGold Tissue DNA Mini Kit (PeqLab), UltraClean Tissue and Cells DNA Isolation Kit (MoBio), DNA Isolation… CONTINUE READING
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