Comparison of cell-based and cell-free protocols for producing target proteins from the Arabidopsis thaliana genome for structural studies.

@article{Tyler2005ComparisonOC,
  title={Comparison of cell-based and cell-free protocols for producing target proteins from the Arabidopsis thaliana genome for structural studies.},
  author={Robert C. Tyler and David J. Aceti and Craig A. Bingman and Claudia C. Cornilescu and Brian G. Fox and Ronnie O. Frederick and Won Bae Jeon and Min Soo Lee and Craig S. Newman and Francis C Peterson and George N. Phillips and Mark N Shahan and Shanteri Singh and Jikui Song and Hassan K. Sreenath and E. H. M. Tyler and Eldon L. Ulrich and Dmitriy A Vinarov and Frank C. Vojtik and Brian F Volkman and Russell L. Wrobel and Qin Zhao and John L. Markley},
  journal={Proteins},
  year={2005},
  volume={59 3},
  pages={633-43}
}
We describe a comparative study of protein production from 96 Arabidopsis thaliana open reading frames (ORFs) by cell-based and cell-free protocols. Each target was carried through four pipeline protocols used by the Center for Eukaryotic Structural Genomics (CESG), one for the production of unlabeled protein to be used in crystallization trials and three… CONTINUE READING