Comparison of bromodeoxyuridine labeling indices obtained from tissue sections and flow cytometry of brain tumors.

@article{Nagashima1988ComparisonOB,
  title={Comparison of bromodeoxyuridine labeling indices obtained from tissue sections and flow cytometry of brain tumors.},
  author={Tadashi Nagashima and Takao Hoshino and Kyung G. Cho and M Senegor and Frederic Waldman and Kazuhiro Nomura},
  journal={Journal of neurosurgery},
  year={1988},
  volume={68 3},
  pages={388-92}
}
Sixteen patients with brain tumors were given a 30- to 60-minute intravenous infusion of bromodeoxyuridine (BUdR), 200 mg/sq m. Grossly viable fragments were taken from the biopsied tumor specimens and divided into two portions. One portion was dissociated into single cells, stained both with fluorescein isothiocyanate (FITC) using anti-BUdR monoclonal antibody as the first antibody and with propidium iodide (for deoxyribonucleic acid), and analyzed by flow cytometry (FCM). The labeling index… CONTINUE READING