Comparison of barley malt alpha-amylase isozymes 1 and 2: construction of cDNA hybrids by in vivo recombination and their expression in yeast.

@article{Juge1993ComparisonOB,
  title={Comparison of barley malt alpha-amylase isozymes 1 and 2: construction of cDNA hybrids by in vivo recombination and their expression in yeast.},
  author={Nathalie Juge and Morten S{\o}gaard and Jean Claude Chaix and Marie France Martin-Eauclaire and Birte Svensson and Guy Marchis-Mouren and Xi-zhi J Guo},
  journal={Gene},
  year={1993},
  volume={130 2},
  pages={
          159-66
        }
}
Germinating barley produces two alpha-amylase isozymes, AMY1 and AMY2, having 80% amino acid (aa) sequence identity and differing with respect to a number of functional properties. Recombinant AMY1 (re-AMY1) and AMY2 (re-AMY2) are produced in yeast, but whereas all re-AMY1 is secreted, re-AMY2 accumulates within the cell and only traces are secreted. Expression of AMY1::AMY2 hybrid cDNAs may provide a means of understanding the difference in secretion efficiency between the two isozymes. Here… CONTINUE READING
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