Comparison of bacteriological qualities of various egg yolk sources and the in vitro and in vivo fertilizing potential of bovine semen frozen in egg yolk or lecithin based diluents.

@article{Bousseau1998ComparisonOB,
  title={Comparison of bacteriological qualities of various egg yolk sources and the in vitro and in vivo fertilizing potential of bovine semen frozen in egg yolk or lecithin based diluents.},
  author={S Bousseau and Jean Pierre Brillard and B Marguant-Le Guienne and Bernard Gu{\'e}rin and A Camus and M Lechat},
  journal={Theriogenology},
  year={1998},
  volume={50 5},
  pages={699-706}
}
The addition of components of animal origin (egg yolk, milk) to most commercial diluents used to freeze bull semen represents a potential risk of contamination of the doses with bacteria or mycoplasma. A series of quantitative and qualitative analyses were performed to detect microbiological contamination observed in Biociphos plus (a new lecithin-glycerol based freezing salt buffer), in an egg yolk diluent (Triladyl) or in an egg yolk + milk-based (Laiciphos) diluent of bull semen. The 2… CONTINUE READING

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