Comparison of bacterial enriched-broth culture, enzyme linked immunosorbent assay, and broth culture-polymerase chain reaction techniques for identifying asymptomatic infections with Salmonella in swine.

@article{Sibley2003ComparisonOB,
  title={Comparison of bacterial enriched-broth culture, enzyme linked immunosorbent assay, and broth culture-polymerase chain reaction techniques for identifying asymptomatic infections with Salmonella in swine.},
  author={Jennifer Sibley and Binbin Yue and Fei Huang and John Harding and Jill Kingdon and Manuel Chirino-Trejo and Greg D. Appleyard},
  journal={Canadian journal of veterinary research = Revue canadienne de recherche veterinaire},
  year={2003},
  volume={67 3},
  pages={219-24}
}
A polymerase chain reaction (PCR) assay was combined with a broth-culture enrichment system to detect Salmonella shed in feces from subclinically infected swine. The effectiveness of the broth culture-polymerase chain reaction (BC-PCR) assay to identify pigs shedding Salmonella in feces was compared with a microbiological culture and a commercial enzyme linked immunosorbent assay (ELISA) kit to detect Salmonella-specific serum antibody. A total of 67 pigs were tested by each of the 3… CONTINUE READING

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