Comparison of antigen contents in co-cultures by an in situ immunoradiometric assay.

Abstract

A fast, sensitive and reproducible in situ immunoradiometric assay has been developed to compare relative contents of cellular markers in cultures. This assay is performed directly in the multi-well plate. After methanol fixation, antigens are identified by specific primary antibodies, followed by 125I-protein A. Cell-associated radioactivity is then… (More)

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