Comparison of anti-fading agents used in fluorescence microscopy: image analysis and laser confocal microscopy study.

@article{Longin1993ComparisonOA,
  title={Comparison of anti-fading agents used in fluorescence microscopy: image analysis and laser confocal microscopy study.},
  author={A Longin and Catherine Souchier and Martine Ffrench and P. A. Bryon},
  journal={The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society},
  year={1993},
  volume={41 12},
  pages={1833-40}
}
To ascertain the ability of commercial and home-made anti-fading media to reduce the decrease of fluorescein isothiocyanate (FITC) fluorescence, we studied the bleaching characteristics of FITC-stained Reh 6 cells mounted in buffered glycerol and in anti-fading media. We measured the intensity of fluorescence over time with a confocal laser scanning microscope and a standard epifluorescence microscope coupled to an image analysis system. Most of the anti-fading media effectively retard fading… CONTINUE READING
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