Comparison of a tuberculin interferon-gamma assay with the tuberculin skin test in high-risk adults: effect of human immunodeficiency virus infection.

@article{Converse1997ComparisonOA,
  title={Comparison of a tuberculin interferon-gamma assay with the tuberculin skin test in high-risk adults: effect of human immunodeficiency virus infection.},
  author={Paul J Converse and Stephen L. Jones and Jacqueline Astemborski and David Vlahov and Neil M. H. Graham},
  journal={The Journal of infectious diseases},
  year={1997},
  volume={176 1},
  pages={144-50}
}
A novel, whole blood interferon-gamma (IFN-gamma) assay was evaluated to determine its suitability for detecting Mycobacterium tuberculosis exposure in intravenous drug users with or without human immunodeficiency virus (HIV) infection. Whole heparinized blood was incubated overnight in separate wells with tuberculin purified protein derivative (PPD), saline, and mitogen controls. Levels of IFN-gamma in plasma supernatants were determined by rapid ELISA. Participants were then administered the… CONTINUE READING