Comparison of a new quantitative ompA-based real-Time PCR TaqMan assay for detection of Chlamydia pneumoniae DNA in respiratory specimens with four conventional PCR assays.

@article{Apfalter2003ComparisonOA,
  title={Comparison of a new quantitative ompA-based real-Time PCR TaqMan assay for detection of Chlamydia pneumoniae DNA in respiratory specimens with four conventional PCR assays.},
  author={Petra Apfalter and Wolfgang Barousch and Marion Nehr and Athanasios Makristathis and Birgit Willinger and Manfred Rotter and Alexander M. Hirschl},
  journal={Journal of clinical microbiology},
  year={2003},
  volume={41 2},
  pages={592-600}
}
Chlamydia pneumoniae, an important respiratory pathogen, is difficult to culture, and detection rates by conventional PCRs vary considerably. A new quantitative ompA-based real-time PCR assay based on TaqMan technology for detection of C. pneumoniae in respiratory samples is described, and its performance in terms of sensitivity and reproducibility is compared with those of four published conventional PCRs (one single-step PCR targeting a cloned PstI fragment; two nested PCRs, one targeting the… CONTINUE READING

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