Comparison of Two Methods of RNA Extraction from Formalin-Fixed Paraffin-Embedded Tissue Specimens

@inproceedings{Gouveia2014ComparisonOT,
  title={Comparison of Two Methods of RNA Extraction from Formalin-Fixed Paraffin-Embedded Tissue Specimens},
  author={Gisele Rodrigues Gouveia and Suzete Cleusa Ferreira and Jerenice Esdras Ferreira and Sheila Aparecida Coelho Siqueira and Juliana Pereira},
  booktitle={BioMed research international},
  year={2014}
}
The present study aimed to compare two different methods of extracting RNA from formalin-fixed paraffin-embedded (FFPE) specimens of diffuse large B-cell lymphoma (DLBCL). We further aimed to identify possible influences of variables--such as tissue size, duration of paraffin block storage, fixative type, primers used for cDNA synthesis, and endogenous genes tested--on the success of amplification from the samples. Both tested protocols used the same commercial kit for RNA extraction (the… CONTINUE READING

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Both tested protocols used the same commercial kit for RNA extraction ( the RecoverAll Total Nucleic Acid Isolation Optimized for FFPE Samples from Ambion ) .
Both tested protocols used the same commercial kit for RNA extraction ( the RecoverAll Total Nucleic Acid Isolation Optimized for FFPE Samples from Ambion ) .
Both tested protocols used the same commercial kit for RNA extraction ( the RecoverAll Total Nucleic Acid Isolation Optimized for FFPE Samples from Ambion ) .
The present study aimed to compare two different methods of extracting RNA from formalin - fixed paraffin - embedded ( FFPE ) specimens of diffuse large B - cell lymphoma ( DLBCL ) .
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