Comparison of Storage Conditions for Human Vaginal Microbiome Studies

  title={Comparison of Storage Conditions for Human Vaginal Microbiome Studies},
  author={Guoyun Bai and Pawel Gajer and Melissa Nandy and Bing Ma and Hongqiu Yang and Joyce M. Sakamoto and May Hsieh Blanchard and Jacques Ravel and Rebecca M Brotman},
  booktitle={PloS one},
BACKGROUND The effect of storage conditions on the microbiome and metabolite composition of human biological samples has not been thoroughly investigated as a potential source of bias. We evaluated the effect of two common storage conditions used in clinical trials on the bacterial and metabolite composition of the vaginal microbiota using pyrosequencing of barcoded 16S rRNA gene sequencing and (1)H-NMR analyses. METHODOLOGY/PRINCIPAL FINDINGS Eight women were enrolled and four mid-vaginal… CONTINUE READING
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Vaginal microbiome of reproductive-age women.

Proceedings of the National Academy of Sciences of the United States of America • 2011

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