Comparison of Sanger sequencing, pyrosequencing, and melting curve analysis for the detection of KRAS mutations: diagnostic and clinical implications.

@article{Tsiatis2010ComparisonOS,
  title={Comparison of Sanger sequencing, pyrosequencing, and melting curve analysis for the detection of KRAS mutations: diagnostic and clinical implications.},
  author={Athanasios C Tsiatis and Alexis Norris-Kirby and Roy G Rich and Michael J. Hafez and Christopher D Gocke and James R. Eshleman and Kathleen M Murphy},
  journal={The Journal of molecular diagnostics : JMD},
  year={2010},
  volume={12 4},
  pages={425-32}
}
Mutations in codons 12 and 13 of the KRAS oncogene are relatively common in colorectal and lung adenocarcinomas. Recent data indicate that these mutations result in resistance to anti-epidermal growth factor receptor therapy. Therefore, we assessed Sanger sequencing, pyrosequencing, and melting curve analysis for the detection of KRAS codon 12/13 mutations in formalin-fixed paraffin-embedded samples, including 58 primary and 42 metastatic colorectal adenocarcinomas, 63 primary and 17 metastatic… CONTINUE READING
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