Comparison of Mitochondrial Fluorescent Dyes in Stromal Cells

@article{Buravkov2014ComparisonOM,
  title={Comparison of Mitochondrial Fluorescent Dyes in Stromal Cells},
  author={Sergey V Buravkov and M. V. Pogodina and Ludmila B Buravkova},
  journal={Bulletin of Experimental Biology and Medicine},
  year={2014},
  volume={157},
  pages={654-658}
}
Specific fluorescent mitochondrial dyes JC-1, MitoTracker Red FM, and MitoTracker Red CMXRos were studied on cultures of mesenchymal stromal stem cells from human adipose tissue. Static and dynamic experiments demonstrated the dependence of dye accumulation in the mitochondria and cytoplasm on mitochondrial potential in the presence of oxidative phosphorylation uncoupler CCCP. JC-1 dye demonstrated most pronounced bleaching upon repeated laser exposure even in very low doses, which should be… CONTINUE READING