Comparison of Methods for Measuring Protein Concentration in Venom Samples

@article{Bocian2020ComparisonOM,
  title={Comparison of Methods for Measuring Protein Concentration in Venom Samples},
  author={Aleksandra Bocian and Sonja Sławek and Marcin Jaromin and Konrad Kamil Hus and Justyna Buczkowicz and Dawid Łysiak and Vladim{\'i}r Petrilla and Monika Petrillov{\'a} and Jaroslav Leg{\'a}th},
  journal={Animals : an Open Access Journal from MDPI},
  year={2020},
  volume={10}
}
Simple Summary Snake venom is mostly composed of proteins and peptides, which are of interest to many researchers due to their potential pharmacological properties. Due to their biochemical character, these components are analyzed using proteomic techniques such as electrophoresis, chromatography and mass spectrometry. A very important stage of such studies is the measurement of protein concentration in the sample, which is most often performed by colorimetric methods. In the presented article… 

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References

SHOWING 1-10 OF 56 REFERENCES
Proteome and Peptidome of Vipera berus berus Venom
TLDR
An attempt was made to identify proteins and peptides derived from this species venom by using high resolution two-dimensional electrophoresis and MALDI ToF/ToF mass spectrometry, and all successfully identified peptides belong to the bradykinin-potentiating peptides family.
Proteomic Analyses of Agkistrodon contortrix contortrix Venom Using 2D Electrophoresis and MS Techniques
TLDR
Ten groups of proteins present in the venom are identified, of which phospholipase A2 and metalloprotease and serine proteases constitute the largest groups, and for the first time presence of 5′-nucleotidase in venom was found in this group of snakes.
Metal and Nonprotein Constituents in Snake Venoms
Snake venoms have been extensively studied in the past and, in general, their gross composition has been quite well-established. Approximately 90% of the dry weight of most venoms consists of protein
Quantitative Proteomic Analysis of Venoms from Russian Vipers of Pelias Group: Phospholipases A2 are the Main Venom Components
TLDR
Protein and peptide compositions of venoms from four Vipera species, inhabiting different regions of Russia, showed the greatest similarity among the four venoms studied, which probably reflected close relationship between these species within the “kaznakovi” complex.
Antibacterial properties of snake venom components
TLDR
Thorough investigations have shown that the phospholipases A 2 and l -amino acids oxidases (LAAO) are mainly responsible for the bactericidal properties of snake venoms, and some preliminary research studies suggest that fragments of three-finger toxins (3FTx) are bactericidal.
A Review and Database of Snake Venom Proteomes
TLDR
This review brings together all compositional studies of snake venom proteomes published in the last decade, allowing rapid assimilation and evaluation of evolutionary trends, geographical variation, and possible medical implications.
Comparative analysis of the venom proteome of four important Malaysian snake species
TLDR
Despite the identifications of major proteins in the four snake species, a large number of protein spots from the 2-DE were unidentified even though the spots displayed high-quality MALDI-TOF-MS spectra.
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