Comparison of Methods for Isolating High-Quality RNA from Leaves of Grapevine

  title={Comparison of Methods for Isolating High-Quality RNA from Leaves of Grapevine},
  author={Elizabeth A. R. Tattersall and Ali Ergul and Fadi Alkayal and Laurent Deluc and John C. Cushman and Grant R Cramer},
Am. J. Enol. Vitic. 56:4 (2005) 400 Extraction of high-quality total RNA is essential for the successful application of many molecular techniques, such as reverse transcription polymerase chain reaction (RTPCR), cDNA library construction, and gene expression profiling studies using microarrays. RNA is degraded rapidly by ribonucleases (RNases) and, therefore, must be extracted quickly and efficiently (Sambrook et al. 1989). Denaturing reagents such as phenol (Davies and Robinson 1996) or… CONTINUE READING


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Publications referenced by this paper.
Showing 1-10 of 10 references

Yield and quality of RNA from grape berries at different developmental stages

  • K. Winter, J. Cushman
  • Am . J . Enol . Vitic .
  • 1995

Method for the isolation of high - quality RNA from grape berry tissues without contaminating tannins or carbohydrates

  • W. Hunter
  • Plant Mol . Biol . Rep .
  • 1991

An effective method of DNA isolation from the mature leaves of Gossypium species that contain large amounts of phenolic terpenoids and tannins

  • R. Croteau
  • Prep . Biochem .
  • 1983

Factors affecting the extraction of ribonucleic acid from plant tissues containing interfering phenolic compounds

  • T. Fujita, K. Zhu-Salzman, P. M. Hasegawa, R. A. Bressan
  • Plant Physiol .
  • 1977

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