Comparison of MALDI-TOF MS with HPLC and nucleic acid sequencing for the identification of Mycobacterium species in cultures using solid medium and broth.

@article{Buchan2014ComparisonOM,
  title={Comparison of MALDI-TOF MS with HPLC and nucleic acid sequencing for the identification of Mycobacterium species in cultures using solid medium and broth.},
  author={Blake W Buchan and Katherine M. Riebe and Markus Timke and Markus Kostrzewa and Nathan A. Ledeboer},
  journal={American journal of clinical pathology},
  year={2014},
  volume={141 1},
  pages={25-34}
}
OBJECTIVES The genus Mycobacterium contains over 150 species including pathogenic and nonpathogenic strains. Accurate species level identification can aid in differentiating environmental contamination from true infection, and also can aid in selection of antimicrobial therapy. METHODS We evaluated the performance of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) for the routine identification of clinical isolates of mycobacteria using 2… CONTINUE READING
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