Comparison of ELISA with activity and ligand-binding methods for the determination of thymidylate synthase concentration.

@article{Tolleson1991ComparisonOE,
  title={Comparison of ELISA with activity and ligand-binding methods for the determination of thymidylate synthase concentration.},
  author={William H. Tolleson and M Alibhai and R J Cisneros and R. Bruce Dunlap},
  journal={Bioconjugate chemistry},
  year={1991},
  volume={2 5},
  pages={327-32}
}
The determination of enzyme levels in cellular extracts by active site titrations or by catalytic activity measurements is relevant in both science and medicine. However, these techniques assume that enzymes exhibit the same response in crude sample matrices as they do in the purified state. We report here an example of how an enzyme-linked immunosorbent assay (ELISA) was used to determine the true enzyme concentration which was compared to the effective enzyme concentration obtained by ligand… CONTINUE READING