Comparison of DNA sequencing of the protein A gene polymorphic region with other molecular typing techniques for typing two epidemiologically diverse collections of methicillin-resistant Staphylococcus aureus.

@article{Oliveira2001ComparisonOD,
  title={Comparison of DNA sequencing of the protein A gene polymorphic region with other molecular typing techniques for typing two epidemiologically diverse collections of methicillin-resistant Staphylococcus aureus.},
  author={Duarte C. Oliveira and I Cris{\'o}stomo and Ilda Santos-Sanches and Pierre Major and Carla R Alves and Marta Aires-de-Sousa and Marianne Konkoly Thege and Herminia de Lencastre},
  journal={Journal of clinical microbiology},
  year={2001},
  volume={39 2},
  pages={574-80}
}
The aim of this study was to compare the recently developed typing approach for methicillin-resistant Staphylococcus aureus (MRSA) based on the DNA sequencing of the protein A gene polymorphic region (spaA typing) with a combination of three well-established molecular typing techniques: ClaI-mecA vicinity polymorphisms, ClaI-Tn554 insertion patterns, and SmaI pulsed-field gel electrophoresis (PFGE) profiles. In order to evaluate the applicability of this typing technique in different types of… CONTINUE READING

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