Comparison of DNA extraction kits for PCR-DGGE analysis of human intestinal microbial communities from fecal specimens

@inproceedings{Ariefdjohan2010ComparisonOD,
  title={Comparison of DNA extraction kits for PCR-DGGE analysis of human intestinal microbial communities from fecal specimens},
  author={Merlin W Ariefdjohan and Dennis A Savaiano and Cindy H. Nakatsu},
  booktitle={Nutrition journal},
  year={2010}
}
BACKGROUND The influence of diet on intestinal microflora has been investigated mainly using conventional microbiological approaches. Although these studies have advanced knowledge on human intestinal microflora, it is imperative that new methods are applied to facilitate scientific progress. Culture-independent molecular fingerprinting method of Polymerase Chain Reaction and Denaturing Gradient Gel Electrophoresis (PCR-DGGE) has been used to study microbial communities in a variety of… CONTINUE READING
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