Comparison of Cryofixation and Aldehyde Fixation for Plant Actin Immunocytochemistry: Aldehydes do not Destroy F-actin

@article{Vitha2000ComparisonOC,
  title={Comparison of Cryofixation and Aldehyde Fixation for Plant Actin Immunocytochemistry: Aldehydes do not Destroy F-actin},
  author={Stanislav Vitha and Franti{\vs}ek Balu{\vs}ka and Markus Braun and Jozef {\vS}amaj and Dieter Volkmann and Peter W. Barlow},
  journal={The Histochemical Journal},
  year={2000},
  volume={32},
  pages={457-466}
}
For walled plant cells, the immunolocalization of actin microfilaments, also known as F-actin, has proved to be much trickier than that of microtubules. These difficulties are commonly attributed to the high sensitivity of F-actin to aldehyde fixatives. Therefore, most plant studies have been accomplished using fluorescent phallotoxins in fresh tissues. Nevertheless, concerns regarding the questionable ability of phallotoxins to bind the whole complement of F-actin necessitate further… CONTINUE READING
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The role of actin in root hair morphogenesis : studies with lipochitooligosaccharide as a growth stimulator and cytochalasin as an actin perturbing drug

DD Miller, de Ruijter NCA, T Bisseling, Emons AMC
Plant J • 1999