Comparison of 5 monoclonal antibodies for immunopurification of human butyrylcholinesterase on Dynabeads: KD values, binding pairs, and amino acid sequences.

@article{Peng2015ComparisonO5,
  title={Comparison of 5 monoclonal antibodies for immunopurification of human butyrylcholinesterase on Dynabeads: KD values, binding pairs, and amino acid sequences.},
  author={Hong Peng and Stephen Brimijoin and Anna Hrabovsk{\'a} and Katarina Targosova and Eric Krejci and Thomas A. Blake and Rudolph C. Johnson and Patrick Masson and Oksana Lockridge},
  journal={Chemico-biological interactions},
  year={2015},
  volume={240},
  pages={336-45}
}
Human butyrylcholinesterase (HuBChE) is a stoichiometric bioscavenger of nerve agents and organophosphorus pesticides. Mass spectrometry methods detect stable nerve agent adducts on the active site serine of HuBChE. The first step in sample preparation is immunopurification of HuBChE from plasma. Our goal was to identify monoclonal antibodies that could be used to immunopurify HuBChE on Dynabeads Protein G. Mouse anti-HuBChE monoclonal antibodies were obtained in the form of ascites fluid, dead… CONTINUE READING

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